US Patent 3,465,720, granted September 9, 1969, to Homare Miyazawa, Shoichi Tarui and Masaki Kamada, assignors to Gunze Limited and Takeda Chemical Industries. It describes rearing silkworms germ-free on artificial diet in a sealed flexible plastic bag. The bag is sterilized and kept inflated with sterile air, with humidity at 60-100% and CO2 below 3%. The patent covers bag materials and sizes per larval stage, diet components and amounts, sterilization, growth and cocoon data compared with a control, and 14 claims. Three drawing figures show the isolator apparatus.

US Patent 3,465,721 (granted Sept. 9, 1969) to Homare Miyazawa, Shoichi Tarui, Masaki Kamada and Hiroshi Masuda, assigned to Gunze Limited and Takeda Chemical Industries, describes a method of rearing fourth- and fifth-stage silkworms on shaped artificial diet. A restricted amount of thin-film, rod, granule or strip diet is fed 2 to 4 times a day, with fasting periods between feedings, to improve feed efficiency, reduce labor and make growth uniform. It describes net and sieve arrangements for separating silkworms from feces and left-over diet, gives tables of diet amounts and feeding areas, and presents five examples with comparisons against known rearing methods, followed by seven claims.