US Patent 3,465,720, granted September 9, 1969, to Homare Miyazawa, Shoichi Tarui and Masaki Kamada, assignors to Gunze Limited and Takeda Chemical Industries. It describes rearing silkworms germ-free on artificial diet in a sealed flexible plastic bag. The bag is sterilized and kept inflated with sterile air, with humidity at 60-100% and CO2 below 3%. The patent covers bag materials and sizes per larval stage, diet components and amounts, sterilization, growth and cocoon data compared with a control, and 14 claims. Three drawing figures show the isolator apparatus.